C-peptide as a Means of Measuring Insulin

Published: 1 Peninsula NIHR clinical Research Facility, Peninsula Medical School, Exeter, UK, and clinical Chemistry, princely Devon and Exeter NHS Foundation Trust, Exeter, UK2 Peninsula NIHR Clinical Research Facility, Peninsula Medical School, Exeter, UK3 Clinical Chemistry, purple Devon and Exeter NHS Foundation Trust, Exeter, UKTitle: Stability and Re professionalducibility of a Single- interpreter urinary C-Peptide/Cr ingestine Ratio and its Correlation with 24-h Urinary C-peptide. IntroductionThe saying is a clinical and correlation experiment to determine how unbosom C-peptide is in urine. For every One C-peptide produced, thither is an equal summate of insulin soupcon produced in the pancreatic beta cell when pro insulin splits into insulin. at that placefrom knowing the amount of urinary C-peptide (UCP) present in a 24hrs urine experiment tells us about the homecoming dictate of insulin in the individual. C-peptide is measured rather than insulin be practi se it has a durable half(prenominal) life. Materials and Methods:The research group analyzed UCP using a unremarkable electrochemiluminescence immunoassay in samples obtained from 21 tidy volunteer, the constancy of UCP with preservatives (boric acid, nuclear number 11 azide and sodium carbonate) were matchd as come up as the reproducibility of urinary C-peptide/creatinine ratio (UCPCR) in first- and second- vacuity fasting urines within a 24-h collections. My chit chat:The researchers could lose analyzed C-peptide using some new(prenominal) techniques to suss out and compargon the mellowedest specificity and sensitivity of C-peptide non just change their technique using subroutine electrochemiluminescence immunoassay, method actings much(prenominal) as:1. Two dimensional plagiarise phase/reverse phase high-performance silver-tongued chromatography. 2.N- storage sequence analysis of endoproteinase Lys_C peptide map usine routine 3.5 method on the HP G1005A N- terminal protein sequencing system. 3.Other! methods include: the Adaltis, RIa, the Dako Elisa, the PerkinElmer successions-resolved fluoroimmunoassay and in hold immunochemiluminometric assay4.The researchers did non pay heed if it was the free or bound C-peptide that was measured. ethanol is efficient to tag and sepa step bound and free C-peptideResults:UCPCR stable at get on temperature for 24hrs at 4 degree centigrade for 72hrs2nd void fasting sample median = 0.78Second-void fasting UCPCRs were lower than first-void (median 0.78 vs 1.31, P = 0.0003) and showed less variate (CV 33% vs 52%), as second-void UCPCRs were not influenced by even out food-related insulin secretion. Second-void fasting UCPCR was highly correlated with 24-h UCP (r = 0.8, P = 0.00006). Study participantMy scuttlebutt:1.The research team could have employ a wider prevail of commonwealth, because the larger the size the better the results discount be analyzed. 30 is the rule of thumb when considering sample size. A wider age scat could have been used as well, not just limiting the age between 24-54yrs. for example monitor the C-peptide in foetus or infants drive out show if there are differences and development due to growth or whether there is an acquired or contractable problem. 2.The parameters for healthy patient were not well defined for example if patients are disease free, transplant patients, or if their organs such as the kidneys are working properly. The races of the healthy patients were not conjureed, because there might be genetic variation in regards to insulin metabolism. fodder could in any case play a calibre in insulin production, the eccentric person of food the participant eat were not mentioned or monitored. For example, patients who predominantly eat food high in carbohydrate, their blood sugar tend to rise to excessive levels, which give rise a condition known as insulin resistance, afterward go the rate of insulin production. High levels of C-peptide also are seen with insulin omas (insulin-producing tumors) and may be seen with ! hypokalemia, pregnancy, Cusings syndrome and renal failure. 3.The research team just now looked at the C-peptide in healthy patients. They could have compared data with those of bad patients to see the differences in C-peptide production.

Patients who are case 1 diabetic experience an autoimmune process that often starts in primeval childhood and involves the nearly complete destruction of the beta cells ( elusive in the production of insulin) over time. Eventually, little or no insulin (and C-peptide) is produced. In type 2 diabetics, the patients are insulin resistant so there is a compensatory increase in in sulin production and absolve that potty also lead to beta cell damage. and so the production rate of C-peptide will vary in these dickens different groups of diabetic patients. 4.How the patients fasted was not well defined by the research group, the time limit of fasting can affect the production of C-peptide. also stress is an otherwise factor that can affect C-peptide production and it wasn?t mentioned in the paper. Creatine RatioMy commentary:Different types of creatin occur in the body such as creatine monohydrate and creatine kinase (CK) also known as creatine phosphokinase (CPK). heave of CK is an extension of damage to many other diseases not only limited to problems with C-peptide. For example lowered CK can be an indication of alcoholic liver diseases which can cause fatty change, or macrovesicular steatosis. The research group did not mention the specific type of creatine they measuredIn conclusion, I don?t think the research was adequately conducted. If the rese arch group change magnitude the range of people they! used for the study and employed other techniques to compare specificity and sensitivity of C-peptide, as well as including windburnt patients (type 1 and 2 diabetics) in their study, the values of the reproducibility and stability of C-peptide will subsequently be affected. Work citedhttp://www.clinchem.org/cgi/content/ synopsis/55/11/2035http://www.chem.agilent.com/Library/applications/59653465.pdfhttp://www3.interscience.wiley.com/journal/112468890/abstracthttp://www.clinchem.org/cgi/reprint/54/6/1023.pdf If you desire to get a full essay, army it on our website: BestEssayCheap.com

If you want to get a full essay, visit our page: cheap essay